We frequently post on the Cytobank blog about interesting topics related to both Cytobank and cytometry in general, as well as provide you with updates on the latest features in Cytobank.
Latest Blog Posts
- My First CyTOF Experiment
Having recently joined Cytobank and with little practical experience with the CyTOF, I headed over to the Nolan lab to do my very first CyTOF experiment. Every year, the Nolan lab hosts a phospho-flow course where a small group of interested researchers fly to Stanford to learn how to perform a phospho-flow experiment. During my day there, I used a protocol in development for this course to generate data on the CyTOF and subsequently used DVS Cytobank for analysis. This is a record of my experiences that will hopefully be helpful to those of you just starting out in the world of mass cytometry as well.
Meet the Cytobank Support Team
It’s nice to put faces to names, right? Well, we’re here to introduce you to the Cytobank Support Team, Geoff and Angela. When you email us for support, generally the reply will be from one of us (occasionally you’ll hear from other team members, and we’ll profile them soon!). We also lead one-on-one trainings by WebEx or Skype, make site visits to do in-person trainings, attend conferences, and you can find us insupport chat for real-time assistance.
Mass Cytometry Resources Added to DVS Cytobank
Earlier this year, we announced the availability of SPADE and Dose Response Analysis through DVS Cytobank and Enterprise servers. We’ve recently added a number of resources to the DVS Cytobank landing page, geared at providing useful content to mass cytometry users.
- Cytobank User Stories: Eric Padron, M.D.
New Buffer Compatibility Data Added to the BD FACSelect Resource
We’ve added new datasheets to the BD FACSelect Buffer Compatibility Resource for targets involved in DNA damage (H2AX (pS139) and p53 (pS37)), apoptosis (active caspase-3 and cleaved PARP), and the cell cycle (7-AAD DNA stain). Visit this resource to see and interact with flow data showing how various antibody conjugates stain in different buffer conditions for a number of mouse and human targets.
Transparency of Data Analysis: Insights from the Institute of Medicine
The trend in flow cytometry is a push toward the development of technologies and methods that will enable researchers to mine increasingly more data from each experiment. These efforts will save time, money, and effort and likewise maximize information yield from each valuable sample. As we continue to grow and mine more data from experiments, a need emerges to ensure that analysis tools are built to support these high dimensional datasets.